ENGARUH PENGGUNAAN LARUTAN KUMUR EKSTRAK BUAH NANAS (Ananas comosus L. Merr) TERHADAP PENURUNAN JUMLAH NEUTROFIL DALAM CAIRAN SULKUS GINGIVA PADA PENDERITA GINGIVITIS RINGAN

  • Raissa Febrina
  • Almurdi Almurdi
  • Kosno Suprianto
Keywords: Gingivitis, Ananas comosus L. Merr, bromelain, gingival crevicular fluid, neutrophil

Abstract

Gingivitis is defined as gingival inflammation caused by dental plaque accumulation. Bacteria in the plaque is releasing toxin that would trigger neutrophil to move to gingival sulcus as a main protection. Plaque control mechanically or chemically will be an effective prevention and care for gingivitis. Chemical control is done by mouthwash. Pineapple extract (Ananas comosus L.merr) contains bromelain which has antibacterial and antiinflammation features. The aim of this study was to find the effect of using pineapple-extracted mouthwash (Ananas comosus L. Merr) toward neutrophil count drop in gingival crevicular fluid of mild gingivitis patients. This study was quasy experimental using pre-test and post-test design, conducted to 21 patients having mild gingivitis with gingival crevicular fluid were taken by paperpoint 30 for 30 seconds. The neutrophil count was done with microscope. Paired T-test analysis showed that there was significant reduction of neutrophil count before and after the intervention (p<0.05). Mouthwash using 50 % pineapple-extracted solution for four days was proven to reduce neutrophil count in gingival crevicular fluid of mild gingivitis patients.

References

1. Bennadi, D. dan C.V.K. Reddy. Oral Health Related Quality of Life. J Int Soc Prev Community-Dent. 2013; 3(1): 1-6.
2. World Health Organization (WHO). Oral Health.http://www.who.int/iris/bitstream/10665/92455/1/9789241506090. 2012. Diakses 30 Oktober 2017.
3. Kementerian Kesehatan Republik Indonesia, Lembaga Penelitian dan Pengembangan Kesehatan Nasional. Laporan Hasil Riset Kesehatan Dasar (Riskesdas). Kementerian Kesehatan 2013. 2013, pp. 10-11.
4. Larasati, R., Hubungan Kebersihan Mulut Dengan Penyakit Sistemik dan Usia Harapan Hidup. Jurnal Skala Husada. 2012; 9(1): 97-104.
5. Kementrian Kesehatan Republik Indonesia. Rencana Program Pelayanan Kesehatan Gigi dan Mulut. 2012, pp. 5-6.
6. Newman, M.G., et al., Carranza’s Clinical Periodontology. 12th Edition. Elsevier Saunders. 2015, pp. 82-87, 120-122, 124-135, 574-577, dan 582-595.
7. Leid, J.G. Bacterials Biofilms Resist Key Host Defense. Microbe. 2009; 4(2): 66-70.
8. Devina, R.I., R. Lessang dan S.L.C. Masulili. Efek Obat Kumur yang Mengandung Temulawak (Curcuma Xanthorrhiza Roxb.) terhadap Gingivitis secara Klinis. The third National Scientific Seminar in Periodontics, Fakultas Kedokteran Gigi Indonesia. 2014.
9. Samaranayake, L. Essential Microbiology for Dentistry. 4th Edition. Churchill Livingstone Elsevier. 2011.
10. Cope, G., Gingivitis: Symptoms, Causes and Treatment. Journal Dental Nursing. 2011; 7(8): 436-439.
11. Turvey, S.E. dan D.H. Broide. Chapter 2: Innate Immunity. J Allergy Clin Immunol. 2010; 125(2 Suppl 2): S24-S32.
12. Baratawidjaja, K.G. dan I. Rengganis. Imunologi Dasar. Edisi Ke-11 (Cetakan ke-2). Fakultas Kedokteran Gigi Indonesia. 2014, pp. 59-60 dan 76-77.
13. Warrington, R., W. Watson, H.L. Kim dan F.R. Antonetti. An Introduction to Immunology and Immunopathology. Pratical Guide for Allergy and Immunology in Canada. 2011; 7(Suppl 1): S1.
14. Rosales, C., N. Demaurex, C.A. Lowell dan E. Uribe-Querol. Neutrophils: Their Role in Innate and Adaptive Immunity. Journal of Immunology Research. 2 pages. 2016.
15. Barros, S.P., R. Williams, S. Offenbacher dan T. Morelli. Gingival Crevicular as a Source of Biomarkers for Periodontitis. Periodontol 2000. 2016; 70(1): 53-64.
16. Rahnama, M., et al., Gingival Crevicular Fluid-Composition and Clinical Importance in Gingivitis and Periodontitis. Journal Public Health. 2014; 124(2): 96-98.
17. Keadaan Jaringan Periodontal. Maj Kedokteran Gigi 17(1): 81-86.
18. Haida, K.E., Cholil, Didit dan Aspriyanto. Perbandingan Efektifitas Mengunyah Buah Pir dan Bengkuang terhadap Penurunan Indeks Plak. Dentino Jurnal Kedokteran Gigi. 2014; 2(1):25.
19. Warongan, M.S.J., P.S. Anindita dan C.N. Mintjelungan. Perbedaan Indeks Plak Penggunaan Obat Kumur Beralkohol dan Non Alkohol pada Penggunaan Obat Ortodontik Cekat. Jurnal e-GiGi (eG). 2015; 3(2): 528.
20. Eley, B.M., M. Soory dan J.D. Manson. Periodontics. 6th Edition. Missouri: Elsevier Saunders. 2010, pp. 19-28.
21. Budiman, I. dan T. Destina. Efek Nanas (Ananas comosus (L.) Merr.) terhadap Penurunan Tekanan Darah. Jurnal Universitas Kristen Maranatha. 2014.
22. Pavan, R., S. Jain, Shraddhah dan A. Kumar. Properties and Therapeutic Application of Bromelain: A Review. Biotechnol Res Int 2012(2012): PMC976203.
23. Ellyfas, K., O.D. Suprobowati dan Djoko SSBU. Pengaruh Pemberian Ekstrak Buah Nanas (Ananas comosus (L.) Merr.) terhadap Kematian Larva Aedes aegypti. Analis Kesehatan Sains 1(2): ISSN 2302-3635. 2012.
24. Silaban, I. dan S. Rahmanisa. Pengaruh Enzim Bromelin Buah Nanas (Ananas comosus L.) terhadap Awal Kehamilan. Majority. 2016; 5(4): 80.
25. Setiawan, B. Daya Hambat Konsentrasi Enzim Bromelin dari Ekstrak Bonggol Nanas (Ananas comosus (L.) Merr) terhadap Streptococcus sanguis. Fakultas Kedokteran Gigi Universitas Hasanuddin. 2016.
26. Novitasari, N.A. Daya Antibakteri Ekstrak Buah Nanas (Ananas comosus) terhadap Pertumbuhan Bakteri Pophyromonas Gingivalis (Tinjauan pada Penerapan Alat Ortodontik Cekat). Perpustakaan Yogyakarta: Fakultas Muhammadiyah Yogyakarta. 2016.
27. Mondal, S., S. Bhattacharya, J.N. Pandey dan M. Biswas. Evaluation of Acute Anti-inflammatory Effect of Ananas Comosus Leaf Extracts in Rats. Pharmacologyonline. 2011; 3: 1312-1315.
28. Hidayat, R. Efek analgesik dan Anti-inflamasi Jus Buah Nanas (Ananas comosus L.) pada Mencit Betina Galur Swiss. Fakultas Farmasi Universitas Sanata Dharma. 2010.
29. Male, D., J. Brostoff, D.B. Roth dan I. Roitt. Immunology. 7th Edition. Mosby Elsevier. 2006.
30. Subowo. Imunologi Klinik. Edisi ke-2. Jakarta: CV Sagung Seto. 2013, pp. 18-23.
31. Pierce, M. dan R. Hardy. Commentary: The Decresing Age of Puberty- as much a Psychosocial as Biological Problem. International Journal of Epidemiology. 2011; 41: 300-302.
32. Laine, M.A. Effect of Pregnancy on Periodontal and Dental Health. Acta Odontol Scand. 2002; 60(5): 257-264.
33. Ali, A., M.A. Milala dan I.A. Gulani. Antimicrobial Effects of Crude Bromelin Extracted from Pineapple Fruit (Ananas comosus (Linn.) Merr.). Advances in Biochemistry. 2015; 3(1): 27.
34. Praveen, N.C., et al., In vitro Evaluation of Antibacterial Efficacy of Pineapple Extract (Bromelain) on Periodontal Pathogens. Journal of International Oral Health. 2014; 6(5): 96-98.
35. Putu, N.M.N., D.M.C. Robin dan M. Syafriadi. Respon Limfosit T Sitotoksik pada Gingivitis Setelah Pemberian Kurkumin (Citotoxic T Lymphocytes Response in Gingivitis After Curcumin Given. Jurnal Pustaka Kesehatan. 2014; 2(1): 43-47.
36. Sudjarwo, S.A., Sinyal Transduksi dari Bromelain sebagai Antiinflamasi pada Udema Telapak Kaki Tikus yang Disebabkan oleh Karagen. Jurnal Kedokteran Brawijaya. 2005; XXI(1): 1-5.
37. Dong, W.L., Efficiacy of Tootbrushing versus an Essential Oil Mouthrinses on Natural Dental Plaque Biofilm in vitro. Journal of Oral Science Research. 2005; 25(3): 288-291.
38. Anggraeni, C.H. Efektivitas Berkumur dengan Larutan Sari Buah Mengkudu (Morinda citrifolia) 100% terhadap Keradangan Gingiva. Disertasi. Universitas Indonesia. 2005.
39. Develas, D., H. Sunarto dan F.M. Tadjoedin. Efek Obat Kumur yang Mengandung Syzygium aromaticum terhadap Gingivitis Secara Klinis. Naskah publikasi. Fakultas Kedokteran Gigi Universitas Indonesia. 2013.
40. Putri, R.D. dan I. Andriani. Daya Antibakteri Ekstrak Kulit Buah Nanas (Ananas comosus) terhadap Pertumbuhan Bakteri Aggregatibacter actinomycetemicomitans. Naskah publikasi. Kedokteran Gigi Universitas Muhammadiyah Yogyakarta. 2016.
41. Ghozali, M.A.Z. Pengaruh Daya Antibakteri Ekstrak Kulit Nanas (Ananas comosus) terhadap bakteri Fusobacterium nucleatum Penyebab Gingivitis (In vitro). Naskah publikasi. Universitas Muhammadiyah Malang. 2017.
Published
2013-12-01
How to Cite
Febrina, R., Almurdi, A., & Suprianto, K. (2013). ENGARUH PENGGUNAAN LARUTAN KUMUR EKSTRAK BUAH NANAS (Ananas comosus L. Merr) TERHADAP PENURUNAN JUMLAH NEUTROFIL DALAM CAIRAN SULKUS GINGIVA PADA PENDERITA GINGIVITIS RINGAN. Andalas Dental Journal, 1(2), 163-173. https://doi.org/10.25077/andalas dent. j.v1i2.165